Preparation for the genetic engineering internship

Preparation for the genetic engineering internship

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The thermal cycler

The thermal cycler is used to carry out the polymerase chain reaction (PCR). First the corresponding heating and cooling phases are programmed, then the reaction vessels are placed in a plastic holder and the lid is closed. After completion of the desired program, the removed samples are passed through commercially available PCR column cleaning kits or (if the product of the PCR is not homogeneous) purified via gel electrophoresis in order to isolate the amplified DNA.

Video: How are GMOs Made? The Genetically Modified Hawaiian Papaya Case Study (July 2022).


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